Know-how
The Eurobiotec Brussels team offers 25 years’ experience of industrial cooperation in the field of industrial microbiology and its applications. This experience finds expression in the following areas:
Selection, stabilisation and formulation of industrial strains:
Within the framework of various research agreements, the group has developed selection protocols for natural bacterial or yeast strains for the production of enzymes or metabolites of interest for the implementation of new continuous bioreactors or for the production of enzymatic activities.
Study of the encapsulation of natural or recombining micro-organisms in order to improve the productivity and stability of the strains.
Study of dry formulations (freeze-drying, fluid bed and coating) for the long-term storage of micro-organisms (yeast and bacterial starter cultures).
Implementation of bioprocesses:
Within the framework of bipartite or multipartite conventions with public bodies dependent on Belgium or the European Union, the group has developed enzymatic bioreactors for the trans-esterification of lipids, bioreactors using living cells (bacterial consortiums or Scenedesmus, Chlorella vulgaris) to denitrify drinking water, modify active principles and analyse their conversion with immobilised rec-CYT q hosts, the semi-continuous production of bacteriocins by immobilising the secreting micro-organisms (C.piscicola, L.lactis), the production of hydrolytic enzymes from B.subtilis, the production of lactic acid or the continuous production of cider (S.cerevisiae + Oenococcus oenos).
Purification of proteins and peptides:
establishment of robust protocols:
Starting from strains such as Saccharomyces cerevisiae, Escherichia coli, Pseudomonas putida, Pseudomonas aeruginaso, Streptococcus faecalis and Lactococcus lactis, the group has developed intracellular enzyme purification protocols such as anabolic ornithine carbamoyltransferase, catabolic ornithine carbamoyltransferase, putrescine carbamoyltransferase, carbamate kinases from arginine deiminase and agmatine deiminase pathways, arginine deiminase, agmatine deiminase, arginoninosuccucinate synthase and arginase, with European academic institutions.
For European and North American industries from the pharmaceutical and diagnoses sector, the group has purified amphiphilic proteins (recombining human proapolipoproteins and apolipoproteins) and peptides (recombining human hormones and certain bacterial peptides) from E.coli, L.lactis, B.subtilis, S.cerevisiae and P.Pastoris In collaboration with European industries, the group has produced and implemented lipase from Zoogloea, papain from Papaya latex, lactoferin and lactoperoxydase from bovine milk, bacteriocin from C.piscicola, human q rec-cytochromes produced by E .coli and tryptophan oxydase produced by various pseudomonaceas (Ps.putida, Ps.aeruginosa, Ps.cepacia, etc.).
In conjunction with several European CMOs, the group has contributed to the development of high cellular density propagation protocols fromE.coli, de L.Lactis, de B. subtilis , from S.cerevisiae, P.pastoris and from L.lactis on behalf of third parties.
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Partners include:
Academic:
Institut de Recherche Microbiologique Jean-Marie Wiame, le Laboratoire de Microbiologie de l’ULB (BE), le Laboratoire de Microbiologie de la Faculté Universitaire des Sciences Agronomiques de Gembloux (BE), ex-Centre de Recherche Industrielle Université Libre de Bruxelles, IRBHN-Fac. Médecine de l’Université Libre de Bruxelles, Laboratoire des protéines Université de Liège (BE), Ecole Nationale d’Ingénieurs des Techniques des Industries Agricoles et Alimentaires (FR), University of Startclyde (G-B), Center for Research and Technology Hellas/Chemical Process Engineering Research Institute (GR), Fraunhoffer Institute (DE), Aristote University of Thesalonniki (GR), Federal Agricultural Resaerch Center (DE), University of Westminster (UK), Lund PAB (Sue)…
Industrial:
UCB-Bio (BE), UCB-Pharma (BE), Esperion (E-U), Cerenis Therapeutics (FR), Brucells (BE), Entomed (FR), Neoferm (E-U) , CIBE (BE), Cofido (BE), Petrofina (BE), Belovo (BE), Oleofina, (BE), Viangro (BE), Janssen Research (BE), ex.ACEC (BE), Lonza (ZW), BAC (NL), Henogen (BE), Eurogentec (BE),Stassen (BE), GeniaLab (DE), Artemia (BE), Detry sa (BE), Kitozyme sa (BE)…